While not named directly in the New Testament, Alexander the Great's conquests established the Greek language and culture (koine Greek) as the lingua franca of the Mediterranean world — the very language in which the New Testament was written.
God's providence is visible in history's great movements. Alexander's conquests spread Greek language and culture across the known world, creating the linguistic foundation for the New Testament. When the gospel needed to reach every nation, the language was already in place. God prepares the way centuries in advance. Daniel's prophecy of the 'kingdom of bronze' (Daniel 2:39) and the swift 'goat' (Daniel 8:5-8) are widely understood as referring to Alexander's empire.